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Recombinant Human Carbonic anhydrase 2 Protein (RP00034)

Bioactivityvalidated

Datasheet

Overview

Product nameRecombinant Human Carbonic anhydrase 2 Protein
Catalog No.RP00034
DescriptionRecombinant Human Carbonic anhydrase 2 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ser2-Lys260) of human Carbonic anhydrase II (Accession #NP_000058.1).
Bio-ActivityMeasured by its esterase activity. The specific activity is >840 pmoles/min/μg, as measured with 1 mM 4-Nitrophenyl acetate and 0.1 μg enzyme at 400 nm in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5.
Purity> 90% by SDS-PAGE.
Endotoxin< 0.1 EU/μg of the protein by LAL method.
FormulationLyophilized from a 0.22 μm filtered solution of 20mM Tris, 150mM NaCl, pH 8.0.Contact us for customized product form or formulation.
SpeciesHuman
Calculated MW29.11 kDa
The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. CA2 is a cytosolic enzyme with the highest activity among all known CAs. Mutations in the CA2 gene result in the CA II deficiency syndrome, an autosomal recessive disorder that produces osteopetrosis, renal tubular acidosis and cerebral calcification.
SynonymCA2; CA-II; CAC; CAII; Car2; HEL-76; HEL-S-282; carbonic anhydrase 2; CA-II; CAC; CAII; Car2; HEL-76; HEL-S-282
Expression HostE. coli
TagNo tag
Swiss-Prot
Gene ID
StorageStore at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
ReconstitutionCentrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.

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