Overview
| Product name | Recombinant Human Complement C5A Protein |
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| Catalog No. | RP01128 |
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| Description | Recombinant Human Complement C5A Protein is produced by E. coli expression system. The target protein is expressed with sequence (Thr678-Arg751) of Human Complement C5A (Accession #NP_001726.2) fused with no tag. |
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| Bio-Activity | Measured by its ability to induce N-acetyl-β-D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells. The ED50 for this effect is typically 5-15 ng/mL.
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| Purity | > 94 % by SDS-PAGE. |
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| Endotoxin | <0.1EU/ug of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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| Species | Human |
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| Calculated MW | 8.17 kDa |
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Background
Human Complement 5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as anaphylatoxins. Human C5a has four alpha -helices plus three intrachain disulfide bonds that create a triple loop structure . In serum, proteolytic processing removes the C‑terminal arginine, creating a low activity C5adesArg74 molecule . Human C5a is 60% and 54% aa identical to mouse and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (C5aR/CD88) and a nonsignaling GPCR termed C5L2 . Activation of Cd88 results in neutrophil chemotaxis and endothelial cell activation . It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells.
Product information
| Synonym | C5; CPAMD4; Complement C5; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4; Cleaved into: Complement C5 beta chain; Complement C5 alpha chain; C5a anaphylatoxin; Complement C5 alpha' chain |
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| Expression Host | E. coli |
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| Tag | NO-Tag |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
