Overview
| Product name | Recombinant Human Complement factor D/CFD Protein |
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| Catalog No. | RP01367 |
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| Description | Recombinant Human Complement factor D/CFD Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ile26-Ala253) of human CFD/Adipsin/PFD (Accession #NP_001919.2.) fused with a 6×His tag at the C-terminus. |
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| Bio-Activity | Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5, 5’Dithio-bis (2-nitrobenzoic acid) (DTNB). The specific activity is >452.49 pmol/min/μg.
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| Purity | > 95% by SDS-PAGE. |
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| Endotoxin | <0.1EU/μg |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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| Species | Human |
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| Calculated MW | 25.24 kDa |
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Background
Complement factor D, also known as Adipsin, C3 convertase activator, Properdin factor D and CFD is a secreted protein which belongs to thepeptidase S1 family. CFD/Adipsin contains onepeptidase S1 domain. Complement factor D ( CFD/Adipsin ) is a component of the alternative complement pathway best known for its role in humoral suppression of infectious agents. Complement factor D ( CFD/Adipsin ) has a high level of expression in fat, suggesting a role for adipose tissue in immune system biology. This protein is also a serine protease that is secreted by adipocytes into the bloodstream. Complement factor D ( CFD/Adipsin ) cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway. Complement factor D ( CFD/Adipsin ) is a serine protease that stimulates glucose transport for triglyceride accumulation in fats cells and inhibits lipolysis. Defects in CFD/Adipsin are the cause of complement factor D deficiency (CFD deficiency) which predisposes to invasive meningococcal disease.
Product information
| Synonym | CFD; ADIPSIN; ADN; DF; PFD |
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| Expression Host | HEK293 cells |
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| Tag | C-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
