Recombinant Human DLL4 Protein (RP00312)

Overview

Product name	Recombinant Human DLL4 Protein
Catalog No.	RP00312
Description	Recombinant Human DLL4 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ser27-Pro524) of Human DLL4 (Accession #Q9NR61) fused with a C-hFc tag at the C-terminus.
Purity	> 95% by SDS-PAGE；> 95% by HPLC
Endotoxin	< 1 EU/μg of the protein by LAL method
Formulation	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Species	Human
Calculated MW	81.1 kDa

Delta-like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands. Activates NOTCH1 and NOTCH4. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Essential for retinal progenitor proliferation. Required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types (By similarity). During spinal cord neurogenesis, inhibits V2a interneuron fate.

Product information

Synonym	DLL4; MGC126344; notch ligand DLL4; Delta4; Delta-like 4
Expression Host	HEK293 cells
Tag	C-hFc
Swiss-Prot
Gene ID

Usage guide

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.