Overview
| Product name | Recombinant Human DNER Protein |
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| Catalog No. | RP00233 |
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| Description | Recombinant Human DNER Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Arg29-His637) of human DNER (Accession #NP_620711.3) fused with an Fc, 6×His tag at the C-terminus. |
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| Purity | > 97% by SDS-PAGE. |
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| Endotoxin | < 0.01 EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
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| Species | Human |
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| Calculated MW | 91.12 kDa |
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Background
DNER (Delta/Notch-like EGF-related receptor), also known as BET (brain-specific EGF-like transmembrane protein), is a type I transmembrane glycoprotein of the Notch/Delta family . In the mouse, DNER has been detected as 90, 120 and 150 kDa forms which are probably variably glycosylated .Activator of the NOTCH1 pathway. May mediate neuron-glia interaction during astrocytogenesis.DNER associates with protein tyrosine phosphatase zeta (PTP zeta ), which is the receptor of pleiotrophin (PTN). PTP zeta -PTN-DNER signaling has been implicated in the regulation of neuritogenesis. Expression of DNER in glioblastoma stem-like cells inhibits formation of neurospheres in vitro, while in vivo it induces differentiation and inhibits growth of xenografts, thus acting as a tumor suppressor.
Product information
| Synonym | DNER; UNQ26; bet |
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| Expression Host | HEK293 cells |
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| Tag | C-hFc&His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
