| Product name | Recombinant Human Dkk-1 Protein |
|---|---|
| Catalog No. | RP01343 |
| Description | Recombinant Human Dkk-1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Thr32-His266) of human DKK-1 (Accession #NP_036374.1) fused with 6×His tag at the C-terminus. |
| Bio-Activity | 1.Measured by its binding ability in a functional ELISA.Immobilized Human DKK-1 at 5 μg/mL (100 μL/well) can bind Human LRP-5 with a linear range of 0.027-0.559 μg/mL. 2.Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T1/2 cells. The EC50 for this effect is approximately 95 ng/ml in the presence of 2 μg/mL of Recombinant Human Wnt3a. |
| Purity | > 95% by SDS-PAGE. |
| Endotoxin | <0.1EU/μg |
| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| Species | Human |
| Calculated MW | 26.61 kDa |
| Synonym | DKK1; DKK-1; SK |
|---|---|
| Expression Host | HEK293 cells |
| Tag | C-His |
| Swiss-Prot | |
| Gene ID |
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
|---|---|
| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
* For research use only. Not for therapeutic or diagnostic purposes.
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