Overview
| Product name | Recombinant Human ENPP-1 Protein |
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| Catalog No. | RP02960 |
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| Description | Recombinant Human ENPP-1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Lys98-Asp925) of human ENPP-1 (Accession #NP_006199.2) fused with a 6×His tag at the C-terminus. |
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| Bio-Activity | Measured by its ability to hydrolyze thymidine 5'-monophosphate p-nitrophenyl ester. The specific activity is >63600 pmol/min/μg, as measured under the described conditions.
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| Endotoxin | < 0.01EU/μg |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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| Species | Human |
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| Calculated MW | 95.95 kDa |
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Background
Ectonucleotide pyrophosphatase/phosphodiesterase (ENPP)-1 is a membrane-bound protein that catalyzes the hydrolysis of extracellular nucleoside triphosphates to monophosphate and extracellular inorganic pyrophosphate (ePPi). Mechanical stimulation regulates ENPP-1 expression.
Product information
| Synonym | ENPP1; ARHR2; COLED; M6S1; NPP1; NPPS; PC-1; PCA1; PDNP1 |
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| Expression Host | HEK293 cells |
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| Tag | C-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
