Overview
| Product name | Recombinant Human ERAP2 Protein |
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| Catalog No. | RP03147LQ |
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| Description | Recombinant Human ERAP2 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Lys505) of Human ERAP2 (Accession #NP_071745.1) fused with His tag at the N-terminus. |
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| Bio-Activity | Measured by its ability to cleave the fluorogenic peptide substrate, Arg-7-amido-4-methylcoumarin (Arg-AMC). The specific activity is >50 pmoles/min/μg.
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| Purity | > 95% by SDS-PAGE. |
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| Endotoxin | < 1 EU/μg of the protein by LAL method. |
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| Formulation | Supplied as a 0.22 μm filtered solution in 12.5mM Tris, 75mM NaCl, pH 7.5, 50% glycercol. Contact us for customized product form or formulation. |
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| Species | Human |
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| Calculated MW | 115-125 kDa. |
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Background
Leukocyte-derived arginine aminopeptidase (LRAP), also known as endoplasmic reticulum-aminopeptidase 2 (ERAP2), is the second identified aminopeptidase localized in the in the lumenal side of endoplasmic reticulum (ER) processing antigenic peptides presented to major histocompatibility complex (MHC) class I molecules. It is a 96-amino acid protein with significant homology to placental leucine aminopeptidase and adipocyte-derived leucine aminopeptidase. LRAP preferentially hydrolyzes the basic residues Arg and Lys, and contains the HEXXH(X)18E zinc-binding motif, which is the characteristic of the M1 family of zinc metallopeptidases which also includes PILS/ARTS1/ERAP1 and LNPEP/PLAP. Induced by interferon-gamma, LRAP is able to trim various MHC class I antigenic peptide precursors.
Product information
| Synonym | Endoplasmic reticulum aminopeptidase 2; Leukocyte-derived arginine aminopeptidase; L-RAP; ERAP2; LRAP |
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| Expression Host | HEK293 cells |
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| Tag | N-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -70℃. This product is stable at ≤ -70℃ for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
