Recombinant Human ERK2/MAPK1 Protein (RP00026)

Overview

Product name	Recombinant Human ERK2/MAPK1 Protein
Catalog No.	RP00026
Description	Recombinant Human ERK2/MAPK1 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Met1-Ser360) of human MAPK1 (Accession #NP_002736.3) fused with a GST tag at the N-terminus.
Bio-Activity	Measured by its binding ability in a functional ELISA. Immobilized Human MAPK1 (Catalog: RP00026LQ) at 1 μg/mL (100 μL/well) can bind ERK1/2 Rabbit mAb (Catalog: A4782) with a linear range of 0.017-4.92 ng/mL.
Purity	> 70% by SDS-PAGE.
Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a 0.22 μm filtered solution of 50mM Tris, 150mM NaCl, 0.1mM EDTA, 0.25mM DTT, 0.1mM AEBSF, pH8.0.Contact us for customized product form or formulation.
Species	Human
Calculated MW	66.89 kDa

ERK2 is a protein serine/threonine kinase, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions.

Product information

Synonym	ERK; ERK-2; ERK2; ERT1; MAPK2; P42MAPK; PRKM1; PRKM2; p38; p40; p41; p41mapk; p42-MAPK; MAPK1
Expression Host	E. coli
Tag	N-GST
Swiss-Prot
Gene ID

Usage guide

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.