Overview
| Product name | Recombinant Human Endostatin/COL18A1 Protein |
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| Catalog No. | RP01938 |
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| Description | Recombinant Recombinant Human Endostatin/COL18A1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln1133-Gln1381) of Human Endostatin/COL18A1 (Accession #NP_569712.2) fused with HuFc and His tag at the N-terminus. |
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| Purity | > 95% by SDS-PAGE. |
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| Endotoxin | <0.01EU/ug of the protein by LAL method |
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| Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
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| Species | Human |
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| Calculated MW | 51.03 kDa |
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Background
Endostatin is able to avoke non-uniform response for proliferation, cell mount and migration of entothelial cells, which different endostatin binding characteristic, leads to the assumption that endostatin effect is strongly dependent from endothelial cell type. Furthermore endostatin inhibits angiogenesis and tumor growth in vivo by inducing apoptosis in endothelial cells. The local delivery of endostatin seems to specifically affect tumor-associated microvessels by reduction of the vessel density, diameter and functionality. Tumor cell migration and invasion was greatly reduced in the endostatin treated animals. Endostatin is non-toxic and does not induce aquired drug resistance and has therefor become a potent new therapy strategy in solid neoplasias. This therapy appears to have a high potential not only for the treatment of gliomas, the most common brain tumors, but also in other tumors.
Product information
| Synonym | COL18A1; Collagen alpha-1(XVIII) chain; Cleaved into: Endostatin; Non-collagenous domain 1; NC1 |
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| Expression Host | HEK293 cells |
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| Tag | N-HuFc-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
