| Product name | Recombinant Human Erythropoietin/EPO Protein |
|---|---|
| Catalog No. | RP01380 |
| Description | Recombinant Human Erythropoietin/EPO Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala28-Arg193) of human EPO/MVCD2 (Accession #NP_000790.2) fused with a hFc tag at the C-terminus. |
| Bio-Activity | 1.Measured by its binding ability in a functional ELISA. Immobilized Human EPOR at 1 μg/mL (100 μL/well) can bind Human EPO with a linear range of 0.24-74.89 ng/mL. 2.Measured in a cell proliferation assay using TF1 human erythroleukemic cells. The ED50 for this effect is 2.37-9.48 ng/mL. |
| Purity | > 95% by SDS-PAGE. |
| Endotoxin | <0.1EU/μg |
| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| Species | Human |
| Calculated MW | 44.35 kDa |
| Synonym | EPO; EP; MVCD2 |
|---|---|
| Expression Host | HEK293 cells |
| Tag | C-hFc |
| Swiss-Prot | |
| Gene ID |
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
|---|---|
| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
* For research use only. Not for therapeutic or diagnostic purposes.
