Recombinant Human Estrogen receptor/ESR1 Protein (RP00815)

Overview

Product name	Recombinant Human Estrogen receptor/ESR1 Protein
Catalog No.	RP00815
Description	Recombinant Human Estrogen receptor/ESR1 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Met1-Gln116) of human Estrogen Receptor (Accession #P03372) fused with a 6×His tag at the N-terminus.
Purity	> 95% by SDS-PAGE.
Endotoxin	< 1 EU/μg of the protein by LAL method.
Formulation	Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2Contact us for customized product form or formulation.
Species	Human
Calculated MW	14.38 kDa

Estrogen Receptor is a major ligand--‐activated transcription factor belonging to the nuclear hormone receptor superfamily. Estrogen Receptor is composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but they also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters.

Product information

Synonym	ER; ESR; ESRA; ESTRR; Era; NR3A1; ESR1; ESRα; ESR; ESRA; ESTRR; Era; NR3A1; ER alpha
Expression Host	E. coli
Tag	N-His
Swiss-Prot
Gene ID

Usage guide

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.