Recombinant Human FGF-2/bFGF Protein (RP01042)

Product name	Recombinant Human FGF-2/bFGF Protein
Catalog No.	RP01042
Description	Recombinant Human FGF-2/bFGF Protein is produced by E. coli expression system. The target protein is expressed with sequence (Pro143-Ser288) of human FGF2 (Accession #NP_001997.5).
Bio-Activity	1.Measured by its binding ability in a functional ELISA. Immobilized Human FGF2 at 0.5 μg/mL (100 μL/well) can bind Human GPC3 with a linear range of 7-20 ng/mL. 2.Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts. The ED₅₀ for this effect is typically 0.635-2.54 ng/mL, corresponding to a specific activity of 3.94 × 10⁵~1.57 × 10⁶ units/mg. 3.Recombinant Human VEGFA(40 ng/mL, Cat. RP01162) and bFGF(50 ng/mL) induce mesoderm cells to differentiate into hematopoietic stem and progenitor cells. After 4 days induction, pebbly-like CD43+ hematopoietic stem and progenitor cells appeared in the hematogenic endothelium. 4.The primary neural stem cells were cultured with 20 ng/mL bFGF and observed every 24 h. Results showed that the particle size of the suspended neural stem cells gradually increased.
Purity	> 95% by SDS-PAGE.
Endotoxin	< 1.0 EU/μg of the protein by LAL method.
Formulation	Lyophilized from a 0.22 μm filtered solution of 20mM Tris，150 mM NaCl, pH7.5.Contact us for customized product form or formulation.
Species	Human
Calculated MW	16.41 kDa

Synonym	BFGF; FGF-2; FGFB; HBGF-2; FGF2; FGF-2; FGFB; HBGF-2; Basic FGF; BFGF; fibroblast growth factor 2
Expression Host	E. coli
Tag	No tag
Swiss-Prot
Gene ID

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.