Overview
| Product name | Recombinant Human FGF-3 Protein |
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| Catalog No. | RP01404 |
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| Description | Recombinant Human FGF-3 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Asp28-Arg212（N-Met）) of human FGF-3 (Accession #NP_005238.1) fused with no additional amino acid. |
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| Purity | > 95% by SDS-PAGE. |
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| Endotoxin | Please contact us for more information. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of 25mM Tris, 1M NaCl, 1mM TCEP, pH 8.0 |
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| Species | Human |
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| Calculated MW | 21.05 kDa |
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Background
Fibroblast Growth Factor 3 (FGF-3) belongs to the large FGF family which has at least 23 members. All FGF family members are heparin-binding growth factors with a core 120 amino acid (aa) FGF domain that allows for a common tertiary structure. FGFs are expressed during embryonic development and in restricted adult tissues. They act on cells of mesodermal and neuroectodermal origin to regulate diverse physiologic functions including angiogenesis, cell growth, pattern formation, embryonic development, metabolic regulation, cell migration, neurotrophic effects and tissue repair. Signaling receptors for FGFs are type I transmembrane receptor tyrosine kinases belonging to the Ig superfamily. Four distinct but related classes of FGF receptors, FGF R1, 2, 3, and 4, exist. Through alternative splicing, multiple isoforms for FGF R1, 2 and 3, with distinct ligand recognition profiles, are also generated.
Product information
| Synonym | FGF3; HBGF-3; INT2 |
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| Expression Host | E. coli |
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| Tag | No tag |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
