Overview
Product name | Recombinant Human FGF-9 Protein |
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Catalog No. | RP01710 |
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Description | Recombinant Human FGF-9 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ala2-Ser208) of human FGF-9 (Accession #NP_002001.1) fused with no additional amino acid. |
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Bio-Activity | Measured in a cell proliferation assay using BALB/3T3 mouse fibroblasts. The ED50 for this effect is 2.34-9.34 ng/mL, corresponding to a specific activity of 1.07×105~4.27×105 units/mg.
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Endotoxin | <1EU/μg |
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Formulation | Lyophilized from a 0.22 μm filtered solution of 20mM PB, pH 6.0. |
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Species | Human |
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Calculated MW | 23.31 kDa |
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Background
Fibroblast growth factor 9 (FGF9) also known as Glia-activating factor or Heparin-binding growth factor 9, is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells.
Product information
Synonym | GAF; FGF-9; SYNS3; HBFG-9; HBGF-9; FGF9 |
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Expression Host | E. coli |
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Tag | NO-tag |
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Swiss-Prot | |
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Gene ID | |
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Usage guide
Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.