| Product name | Recombinant Human FGFR-4/CD334 Protein |
|---|---|
| Catalog No. | RP00130 |
| Description | Recombinant Human FGFR-4/CD334 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Leu22-Asp369) of human FGFR4/FGF Receptor 4 (Accession #NP_998812.1) fused with an Fc, 6×His tag at the C-terminus. |
| Bio-Activity | 1.Measured by its binding ability in a functional ELISA. Immobilized recombinant human FGF2 at 1 μg/mL (100 μL/well) can bind recombinant human FGFR4 with a linear range of 30-125 ng/mL. 2.Measured by its binding ability in a functional ELISA. Immobilized recombinant human FGFR4 at 5 μg/mL (100 μL/well) can bind recombinant human FGF12 with a linear range of 35-100 ng/mL. 3.Measured by its ability to inhibit FGF-acidic dependent proliferation of Balb/c 3T3 mouse fibroblasts. The ED50 for this effect is typically 0.03-0.12 ng/mL. |
| Purity | > 95% by SDS-PAGE. |
| Endotoxin | < 0.1 EU/μg of the protein by LAL method. |
| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
| Species | Human |
| Calculated MW | 65.30 kDa |
| Synonym | CD334; JTK2; TKF; FGFR4; JTK2; TKF |
|---|---|
| Expression Host | HEK293 cells |
| Tag | C-hFc&His |
| Swiss-Prot | |
| Gene ID |
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
* For research use only. Not for therapeutic or diagnostic purposes.
