Overview
Product name | Recombinant Human Fetuin A/AHSG Protein |
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Catalog No. | RP00195 |
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Description | Recombinant Human Fetuin A/AHSG Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala19-Val367) of human Fetuin-A/AHSG/FETUA (Accession #NP_001613.2) fused with a 6×His tag at the C-terminus. |
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Bio-Activity | 1.Measured by its ability to inhibit calcium phosphate precipitation. The IC50 value is <25 μg/mL.
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Purity | > 95% by SDS-PAGE. |
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Endotoxin | < 0.1 EU/μg of the protein by LAL method. |
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Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
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Species | Human |
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Calculated MW | 38.18 kDa |
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Background
Fetuin-A, also known as Alpha-2-HS-Glycoprotein (AHSG), belongs to the Fetuin family.It is a major plasma protein and a member of the cystatin superfamily of protease inhibitors.It is expressed by hepatocytes, the principal cell source, and by monocyte/macrophages. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has therefore been postulated that it participates in the development of the tissues.
Product information
Synonym | AHSG; A2HS; AHS; FETUA; HSGA; alpha-2-HS-glycoprotein |
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Expression Host | HEK293 cells |
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Tag | C-His |
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Swiss-Prot | |
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Gene ID | |
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Usage guide
Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.