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Recombinant Human Follistatin/Follistatin 288/FST Protein (RP00376)

Bioactivityvalidated

Datasheet

Overview

Product nameRecombinant Human Follistatin/Follistatin 288/FST Protein
Catalog No.RP00376
DescriptionRecombinant Human Follistatin/Follistatin 288/FST Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly30-Asn317) of human Follistatin/Follistatin 288/FST (Accession #P19883) with a hFC at the N-terminus..
Bio-ActivityMeasured by its ability to neutralize Activin-mediated erythroid differentiation of K562 human chronic myelogenous leukemia cells. The ED50 for this effect is 0.1-0.4 μg/mL in the presence of 7.5 ng/mL of rhActivin A.
Purity> 97% by SDS-PAGE.
Endotoxin< 0.01 EU/μg of the protein by LAL method.
FormulationLyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
SpeciesHuman
Calculated MW54.19 kDa
Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. In a study in which 37 candidate genes were tested for linkage and association with polycystic ovary syndrome (PCOS) or hyperandrogenemia in 150 families, evidence was found for linkage between PCOS and follistatin.
SynonymFST; FS
Expression HostHEK293 cells
TagN-hFC
Swiss-Prot
Gene ID
StorageStore at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
ReconstitutionCentrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.

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