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Recombinant Human Frizzled-7/FZD7 Protein (RP00204)

Bioactivityvalidated

Datasheet

Overview

Product nameRecombinant Human Frizzled-7/FZD7 Protein
Catalog No.RP00204
DescriptionRecombinant Human Frizzled-7/FZD7 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln33-Leu185) of human Frizzled-7 (Accession #NP_003498.1) fused with an Fc, 6×His tag at the C-terminus.
Bio-Activity1.Measured by its binding ability in a functional ELISA. Immobilized Human Glypican-3 Protein at 5 μg/mL (100 μL/well) can bind Human FZD7 with a linear range of 4.88-935.5 ng/mL.
2.Measured by its binding ability in a functional ELISA. Immobilized Human Glypican-3 Protein at 5 μg/mL (100 μL/well) can bind Human FZD7 with a linear range of 4.88-520.4 ng/mL.
Purity> 95% by SDS-PAGE.
Endotoxin< 0.1 EU/μg of the protein by LAL method.
FormulationLyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
SpeciesHuman
Calculated MW43.49 kDa
Frizzled-7 is a member of the Frizzled family of unconventional G-protein-coupled glycoprotein receptors for the Wnt signaling pathway. During development, Frizzled-7 is expressed during gastrulation and in the fetal gut, kidney and lung where it is thought to influence tissue morphogenesis via non-canonical signaling pathways . In the adult, Frizzled-7 is expressed in skeletal muscle, especially in satellite cells that mediate muscle regeneration in response to Wnt-7a. It is expressed in embryonic stem cells (ES), contributing to self-renewal signaling . Frizzled-7 expression may downregulate APC function and enhance beta-catenin-mediated signals in poorly differentiated human esophageal carcinomas.
SynonymFZD7; FzE3
Expression HostHEK293 cells
TagC-hFc&His
Swiss-Prot
Gene ID
StorageStore at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
ReconstitutionCentrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.

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