Recombinant Human GTPase KRas/KRAS(G12V) Protein (RP01239)

Product name	Recombinant Human GTPase KRas/KRAS(G12V) Protein
Catalog No.	RP01239
Description	Recombinant Human GTPase KRas/KRAS(G12V) Protein is produced by E. coli expression system. The target protein is expressed with sequence (Thr2-Cys186(Gly12Val)) of human GTPase KRas/KRAS (Accession #NP_203524.1) fused with a 6×His tag at the N-terminus.
Bio-Activity	Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human KRAS, His Tag at 2 μg/mL (100 μL/well) can bind Anti-KRAS Ab with a linear range of 42-84.56 ng/mL.
Purity	> 85% by SDS-PAGE.
Endotoxin	< 1.0 EU/μg of the protein by LAL method.
Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Species	Human
Calculated MW	22.04 kDa

Synonym	KRAS; C-K-RAS; CFC2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI-RAS; KRAS1; KRAS2; NS; NS3; RALD; RASK2; c-Ki-ras2; GTPase KRas; C-K-RAS; CFC2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI-RAS; KRAS1; KRAS2; NS; NS3; RALD; RASK2; c-Ki-ras2
Expression Host	E. coli
Tag	N-His
Swiss-Prot
Gene ID

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.