| Product name | Recombinant Human Galectin-9/LGALS9 Protein |
|---|---|
| Catalog No. | RP01046 |
| Description | Recombinant Human Galectin-9/LGALS9 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala2-Thr323) of human Galectin-9/LGALS9 (Accession #NP_002299.2) fused with a 6×His tag at the N-terminus. |
| Bio-Activity | 1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Galectin-9 at 2 μg/mL can bind Recombinant Human TIM-3 with a linear range of 5-15 μg/mL. 2.Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8×104 cells/well are added to LGALS9 coated plates (5 μg/mL and 100 μL/well) in the ppresence of 10μg/ml PHA, approximately 70-80% cells will adhere specifically after 60 minutes at 37℃. |
| Purity | > 92% by SDS-PAGE. |
| Endotoxin | Please contact us for more information. |
| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
| Species | Human |
| Calculated MW | 36.59 kDa |
| Synonym | LGALS9; HUAT; LGALS9A |
|---|---|
| Expression Host | HEK293 cells |
| Tag | N-His |
| Swiss-Prot | |
| Gene ID |
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
|---|---|
| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
* For research use only. Not for therapeutic or diagnostic purposes.
