Overview
| Product name | Recombinant Human Gamma-glutamyl hydrolase/GGH Protein |
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| Catalog No. | RP01957 |
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| Description | Recombinant Human Gamma-glutamyl hydrolase/GGH Protein is produced by E. coli expression system. The target protein is expressed with sequence (Arg25-Asp318) of Human Gamma-glutamyl hydrolase/GGH (Accession #NP_003869.1) fused with a His tag at the N-terminus. |
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| Purity | > 95% by SDS-PAGE. |
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| Endotoxin | < 0.1 EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of 20mM HEPES, 150mM NaCl, 1mM TCEP, pH 8.0. |
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| Species | Human |
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| Calculated MW | 34.93 kDa |
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Background
Gamma-glutamyl hydrolase/GGH regulates intracellular folates and antifolates such as methotrexate (MTX) for proper nucleotide biosynthesis and antifolate-induced cytotoxicity, respectively. GGH is a ubiquitously-expressed enzyme that regulates intracellular folate metabolism for cell proliferation, DNA synthesis, and repair. cytoplasmic GGH might play a relevant role in the development and progression of invasive breast cancer.
Product information
| Synonym | GGH; Gamma-glutamyl hydrolase; EC:3.4.19.9; Conjugase; GH; Gamma-Glu-X carboxypeptidase |
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| Expression Host | E. coli |
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| Tag | N-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
