Overview
| Product name | Recombinant Human Guanylyl cyclase C/GUCY2C Protein |
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| Catalog No. | RP01430 |
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| Description | Recombinant Human Guanylyl cyclase C/GUCY2C Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ser24-Gln430) of human GUCY2C/Guanylyl cyclase (Accession #NP_004954.2) fused with a 6×His，Avi tag at the C-terminus. |
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| Bio-Activity | Measured by its binding ability in a functional ELISA. Immobilized Human GUCY2C at 1 μg/mL (100 μL/well) can bind GUCY2C Rabbit pAb with a linear range of 1-144 ng/mL.
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| Purity | > 95% by SDS-PAGE. |
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| Endotoxin | Please contact us for more information. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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| Species | Human |
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| Calculated MW | 48.66 kDa |
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Background
GUCY2C (Guanylyl Cyclase C), also known as heat-stable enterotoxin receptor, is a type I transmembrane protein of the guanylate cyclase (gc) family that signal by producing cGMP. Guanylate cyclase C (GUCY2C) and its hormones guanylin and uroguanylin have recently emerged as one paracrine axis defending intestinal mucosal integrity against mutational, chemical, and inflammatory injury. GUCY2C murine CAR-T cells recognized and killed human colorectal cancer cells endogenously expressing GUCY2C. Thus, we have identified a human GUCY2C-specific CAR-T cell therapy approach that may be developed for the treatment of GUCY2C-expressing metastatic colorectal cancer. GUCY2C may represent a new target for anti-obesity pharmacotherapy.
Product information
| Synonym | GUCY2C; DIAR6; GC-C; GUC2C; MECIL; MUCIL; STAR |
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| Expression Host | HEK293 cells |
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| Tag | C-His&Avi |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
