Overview
| Product name | Recombinant Human HGF Activator/HGFA Protein |
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| Catalog No. | RP03236 |
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| Description | Recombinant Human HGF Activator/HGFA Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln36-Ser655) of Human HGF Activator/HGFA (Accession #NP_001519.1) fused with His tag at the C-terminus. |
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| Purity | ≥ 95 % as determined by SDS-PAGE |
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| Endotoxin | < 0.01 EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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| Species | human |
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| Calculated MW | 68.2 kDa |
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Background
HGF activator (HGFA) is a serum-derived serine protease and belongs to the peptidase family S1.HGFA is responsible for the conversion of hepatocyte growth factor (HGF), from the inactive single-chain precursor to the active heterodimeric form, which is a potent mitogen, motogen, and morphogen for liver cells, epithelial cells, and endothelial cells. HGFA is synthesized and secreted by the liver and circulates in the plasma as an inactive single-chain zymogen in normal states. The zymogen is cleaved by thrombin or thermolysin through the endoproteolytic process and forms an active heterodimer linked by a disulfide bond. In turn, the active protease can be inhibited by HGFA inhibitors (HAIs) including HAI-1 and HAI-2. Besides, the HGFA zymogen acquires a strong affinity upon activation and thus may ensure the local action in tissue regeneration in the liver, kidney, and skin. It has been reported that activation of HGF is a critical limiting step in the HGF/SF-induced signaling pathway mediated by Met, and accordingly, aberrant expression of HGFA is implicated in tumorigenesis and progression.
Product information
| Synonym | HGFAC; Hepatocyte growth factor activator; HGF activator; HGFA; EC:3.4.21.-; Cleaved into: Hepatocyte growth factor activator short chain; Hepatocyte growth factor activator long chain |
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| Expression Host | HEK293 cells |
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| Tag | C-6His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
