Product name | Recombinant Human Hepatocyte growth factor/HGF Protein |
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Catalog No. | RP01602 |
Description | Recombinant Human Hepatocyte growth factor/HGF Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln32-Ser728) of human Hepatocyte growth factor/HGF (Accession #NP_000592.3) fused with and a 6×His tag at the C-terminus. |
Bio-Activity | Measured by its ability to induce IL-11 secretion by Saos-2 human osteosarcoma cells. Hjertner, O. et al. (1999) Blood 94:3883. The ED50 for this effect is 2.23-8.91 ng/mL, corresponding to a specific activity of 1.12×105-4.48×105 units/mg. |
Purity | > 95% by SDS-PAGE. |
Endotoxin | <0.1EU/μg |
Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
Species | Human |
Calculated MW | 80.51 kDa |
Synonym | HGF; DFNB39; F-TCF; HGFB; HPTA; SF |
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Expression Host | HEK293 cells |
Tag | C-His |
Swiss-Prot | |
Gene ID |
Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
* For research use only. Not for therapeutic or diagnostic purposes.
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