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Recombinant Human Hepatocyte growth factor/HGF Protein (RP01602)

Bioactivityvalidated

Review (2) Datasheet

Overview

Product nameRecombinant Human Hepatocyte growth factor/HGF Protein
Catalog No.RP01602
DescriptionRecombinant Human Hepatocyte growth factor/HGF Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln32-Ser728) of human Hepatocyte growth factor/HGF (Accession #NP_000592.3) fused with and a 6×His tag at the C-terminus.
Bio-ActivityMeasured by its ability to induce IL-11 secretion by Saos-2 human osteosarcoma cells. Hjertner, O. et al. (1999) Blood 94:3883. The ED50 for this effect is 2.23-8.91 ng/mL, corresponding to a specific activity of 1.12×105-4.48×105 units/mg.
Purity> 95% by SDS-PAGE.
Endotoxin<0.1EU/μg
FormulationLyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
SpeciesHuman
Calculated MW80.51 kDa
Hepatocyte growth factor/scatter factor (HGF/SF) is a paracrine cellular growth, motility and morphogenicfactor. It belongs to the peptidase S1 family and Plasminogen subfamily, contains 4 kringle domains, 1 PANdomain and 1 peptidase S1 domain. HGF regulates cell growth, cell motility, and morphogenesis by activatinga tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. HGF is secreted bymesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability tostimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration.
SynonymHGF; DFNB39; F-TCF; HGFB; HPTA; SF
Expression HostHEK293 cells
TagC-His
Swiss-Prot
Gene ID
StorageStore at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
ReconstitutionCentrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.

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16 Sep 2024-20 Dec 2024 on sale

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