Recombinant Human ICAM-1/CD54 Protein (RP00272)

Overview

Product name	Recombinant Human ICAM-1/CD54 Protein
Catalog No.	RP00272
Description	Recombinant Human ICAM-1/CD54 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Gln28-Glu480) of human ICAM-1/CD54 (Accession #NP_000192.2) fused with a 6×His tag at the C-terminus.
Bio-Activity	Measured by its binding ability in a functional ELISA.Immobilized APC Mouse Anti-Human CD54 at 1μg/mL (25 μL/well) can bind Human CD54 with a linear range of 0.46-7.8 ng/mL.
Purity	> 95% by SDS-PAGE.
Endotoxin	< 0.1 EU/μg of the protein by LAL method.
Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Species	Human
Calculated MW	50.34 kDa

This protein is a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation.

Product information

Synonym	BB2; CD54; P3.58; ICAM1
Expression Host	HEK293 cells
Tag	C-His
Swiss-Prot
Gene ID

Usage guide

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.