Overview
| Product name | Recombinant Human IFN-alpha 4 Protein |
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| Catalog No. | RP01914 |
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| Description | Recombinant Recombinant Human IFN-alpha 4 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Cys24-Asp189) of Human IFN-alpha 4(Accession #NP_066546.1) fused with a His tag at the C-terminus. |
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| Bio-Activity | Measured in a cell cytotoxicity assay using TF-1 cells. The ED50 for this effect is 0.26‑1.02 ng/mL, corresponding to a specific activity of 9.80×105~3.85×106 units/mg.
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| Purity | > 90% by SDS-PAGE. |
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| Endotoxin | < 0.1EU/μg of the protein by LAL method |
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| Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
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| Species | Human |
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| Calculated MW | 20.22 kDa |
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Background
IFN-alpha 4, Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.The interferons (IFN) are a family of cytokines with potent antiviral, antiproliferative and immunomodulatory properties, and are classified based on their binding specificity to cell surface receptors . The type I IFNs bind to the interferon alpha receptor (IFNAR), which consists of two subunits: IFNAR1 ( alpha -subunit) and IFNAR2 ( beta -subunit). This binding contributes to TNF-alpha induced signaling . Both the human and mouse genome code for more than a dozen closely related IFN-alpha subtypes and the various IFN-alphas share about 80% sequence homology among them . The mouse IFN-alpha 4 consists of 186 amino acids (aa) including a 24 aa signal peptide and a 162 aa IFN-alpha 4 mature domain. The mature mouse IFN-alpha 4 shares 58% and 76% sequence identity with mature human and rat IFN-alpha 4, respectively. The mouse IFN-alpha 4 reduces the local replication of murine cytomegalovirus in the tibia muscle. mIFN-A4 is a strong activator of Mx gene and was shown to be very effective in reducing splenomegaly .
Product information
| Synonym | Interferon alpha-4; IFN-alpha-4; Interferon alpha-4B; Interferon alpha-76; Interferon alpha-M1; IFNA4 |
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| Expression Host | HEK293 cells |
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| Tag | C-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
