Overview
| Product name | Recombinant Human IFN-alpha H2/IFNA14 Protein |
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| Catalog No. | RP01897 |
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| Description | Recombinant Human IFN-alpha H2/IFNA14 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Cys24-Asp189) of Human IFN-alpha H2/IFNA14 (Accession #NP_002163.2) fused with a His tag at the C-terminus. |
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| Bio-Activity | Measured in a cell cytotoxicity assay using TF-1 cells. The ED50 for this effect is 0.47‑1.88 ng/mL, corresponding to a specific activity of 5.32×105~2.13×106 units/mg.
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| Purity | > 95% by SDS-PAGE. |
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| Endotoxin | <0.01EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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| Species | Human |
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| Calculated MW | 20.55 kDa |
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Background
Interferons (IFN) are a family of cytokines with potent antiviral, antiproliferative and immunomodulatory properties, classified based on their binding specificity to cell surface receptors . Human IFNA2 was originally cloned in the early ‘80s and now more than a dozen closely related IFN alpha subtypes have been identified in both the human and mouse genome, each sharing about 80% amino acid (aa) sequence homology . Structurally, type I IFNs belong to the class of five helical bundle cytokines, with the IFNA subtypes containing 2 conserved disulfide bonds. The extracellular domain (ECD) of mature human IFNA14, shares 58% aa sequence identity with mouse IFNA14. The type I IFNs bind to the interferon alpha receptor (IFNAR), which consists of two subunits: IFNAR1 (alpha subunit) and IFNAR2 (beta-subunit). Individual IFNA subtypes are known to display unique efficacies to viral protection, and IFNA14 has been shown to be a strong inducer of IFN-stimulated genes and anti-viral protection . IFNA14 has been shown to be potent against HIV-1 by up regulating the transcription of two intrinsic restriction factors with well-established anti-HIV-1 activity, MX2 and tetherin.
Product information
| Synonym | Interferon alpha-14; IFN-alpha-14; Interferon alpha-H; LeIF H; Interferon lambda-2-H，IFNA14 |
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| Expression Host | HEK293 cells |
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| Tag | C-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
