Overview
| Product name | Recombinant Human IFN-alpha WA/ IFNA16 Protein |
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| Catalog No. | RP01921 |
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| Description | Recombinant Human IFN-alpha WA/ IFNA16 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Cys24-Asp189) of Human IFN-alpha WA/ IFNA16 (Accession #NP_002164.1) fused withHis tag at the C-terminus. |
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| Endotoxin | <1EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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| Species | Human |
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| Calculated MW | 20.12 kDa |
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Background
Interferons (IFN) are a family of cytokines with potent antiviral, antiproliferative and immunomodulatory properties, classified based on their binding specificity to cell surface receptors . Human IFNA2 was originally cloned in the early ‘80s and now more than a dozen closely related IFN alpha subtypes have been identified in both the human and mouse genome, each sharing about 80% amino acid (aa) sequence homology . Structurally, type I IFNs belong to the class of five helical-bundle cytokines, with the IFNA subtypes containing 2 conserved disulfide bonds . Mature human IFNA16 shares 60% aa sequence identity with mouse IFNA16. The type I IFNs bind to the interferon alpha receptor (IFNAR), which consists of two subunits: IFNAR1 (alpha -subunit) and IFNAR2 (beta -subunit). Individual IFNA subtypes are known to display unique efficacies to viral protection, and IFNA16 has been shown to be an intermediate inducer of IFN-stimulated genes and anti-viral protection . IFNA16 has been shown to be upregulated during chronic HIV infection and might play a role in lipid accumulation and fatty acid deposition in porcine muscle .
Product information
| Synonym | BC114392; Gm13280; IFNA16; Ifna6T; IFN-alpha 16; IFNalpha WA; IFN-alpha WA; IFN-alpha-16; IFN-alpha-N-protein; IFN-alphaO; IFN-alpha-WA; Ifnat6; interferon alpha-16; Interferon Alpha-WA; interferon; alpha 16 |
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| Expression Host | HEK293 cells |
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| Tag | C-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
