Overview
| Product name | Recombinant Human IGFBP-2 Protein |
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| Catalog No. | RP00215 |
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| Description | Recombinant Human IGFBP-2 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Glu40-Gln328) of human IGFBP-2 (Accession #NP_000588.2) fused with an initial Met at the N-terminus and a 6×His tag at the C-terminus. |
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| Bio-Activity | Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human IGFBP-2 at 5 μg/mL (100 μL/well) can bind Recombinant Human IGF1 with a linear range of 53-212 ng/mL.
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| Purity | > 95% by SDS-PAGE. |
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| Endotoxin | < 1.0 EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
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| Species | Human |
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| Calculated MW | 32.22 kDa |
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Background
IGFBP-2, also known as IGFBP2, is a insulin-like growth factor-binding protein (IGFBP). IGFBPs have a high affinity for IGFs. Some members of the IGFBP family have been consistently shown to inhibit IGF actions by preventing them from gaining access to the IGF receptors, while others potentiate IGF actions by facilitating the ligand-receptor interaction. IGFBP2 is overexpressed in many malignancies and is often correlated with an increasingly malignant status of the tumor, pointing to a potential involvement of IGFBP2 in tumorigenesis.
Product information
| Synonym | IBP2; IGF-BP53; IGFBP2; IGF-BP53 |
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| Expression Host | HEK293 cells |
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| Tag | C-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
