Recombinant Human IL-17A/CTLA-8 Protein (RP00212)

Overview

Product name	Recombinant Human IL-17A/CTLA-8 Protein
Catalog No.	RP00212
Description	Recombinant Human IL-17A/CTLA-8 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ile20-Ala155) of human IL-17A (Accession #NP_002181.1) fused with a 6×His tag at the C-terminus.
Bio-Activity	1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant human IL17RA at 2 μg/mL (100 μL/well) can bind Recombinant human IL-17A, the EC₅₀ of human IL-17A is 32.5-130 ng/mL. 2.Measured by its ability to induce IL-6 secretion by Hela cells. The ED₅₀ for this effect is 2.64-10.58 ng/mL, corresponding to a specific activity of 9.45×10⁴~3.79×10⁵ units/mg.
Purity	> 95% by SDS-PAGE.
Endotoxin	< 0.1 EU/μg of the protein by LAL method.
Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Species	Human
Calculated MW	16.37 kDa

IL17, also known as IL17a and CTLA8, is a is a 15-20 kDa glycosylated cytokine which belongs to the IL-17 family. The IL-17 family of cytokines includes six members, IL-17/IL-17A, IL-17B, IL-17C, IL-17D, IL-17E/IL-25, and IL-17F, which are produced by multiple cell types. IL17A promotes protective mucosal and epidermal inflammation in response to microbial infection .It induces chemokine production, neutrophil influx, and the production of antibacterial peptides .IL17A additionally enhances the production of inflammatory mediators by rheumatoid synovial fibroblasts and contributes to TNF alpha induced shock . In contrast, it can protect against the progression of colitis by limiting chronic inflammation . IL17A encourages the formation of autoreactive germinal centers and exacerbates the onset and progression of experimental models of autoimmunity .

Product information

Synonym	IL17A; CTLA-8; CTLA8; IL-17; IL-17A; IL17; interleukin-17A; CTLA-8; CTLA8; IL-17; IL-17A; IL17
Expression Host	HEK293 cells
Tag	C-His
Swiss-Prot
Gene ID

Usage guide

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.