| Product name | Recombinant Human IL-23/IL-12B&IL-23A Protein |
|---|---|
| Catalog No. | RP01160 |
| Description | Active Recombinant Human IL-12B&IL-23A Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ile23-Ser328&Arg20-Pro189) of human IL-23 (Accession #NP_002178.2/NP_057668.1) fused with a 6×His tag at the N-terminus(IL23A). |
| Bio-Activity | 1.Measured by its binding ability in a functional ELISA.Immobilized Human IL-12B&IL-23A at 5μg/mL (100 μL/well) can bind Human IL23R with a linear range of 4-135 ng/ml. 2.Measured by its ability to induce IL-17 secretion by mouse splenocytes. The ED50 for this effect is 21.23-84.94 ng/mL, corresponding to a specific activity of 1.17×104~4.71×104 units/mg. |
| Purity | > 95% by SDS-PAGE. |
| Endotoxin | < 0.1 EU/μg of the protein by LAL method. |
| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
| Species | Human |
| Calculated MW | 19.50 kDa(IL-12A), 34.71 kDa(IL-12B) |
| Synonym | IL-23 p19/IL-12 p40; IL23; SGRF ; IL-23 alpha & IL-12 beta |
|---|---|
| Expression Host | HEK293 cells |
| Tag | No tag(IL12B)&N-his(IL23A) |
| Swiss-Prot | |
| Gene ID |
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
|---|---|
| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
* For research use only. Not for therapeutic or diagnostic purposes.
