Recombinant Human IL-7 Protein (RP01040)

Product name	Recombinant Human IL-7 Protein
Catalog No.	RP01040
Description	Active Recombinant Human IL-7 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asp26-His177) of human IL-7 (Accession #NP_000871.1) fused with a 6×His tag at the C-terminus.
Bio-Activity	1.Measured by its binding ability in a functional ELISA.Immobilized Human IL7 at 2 μg/mL (100 μL/well) can bind human IL7R with a linear range of 0.97 -159.1 ng/mL. 2.Measured in a cell proliferation assay using IL-2(Catalog: RP01384)-activated mouse spleen cell. The ED₅₀ for this effect is 0.1-0.4 ng/mL, corresponding to a specific activity of 2.5×10⁶~1×10⁷ units/mg. 3.Measured in a cell proliferation assay using murine 2E8 cells. The ED₅₀ for this effect is 0.78-3.1ng/mL, corresponding to a specific activity of 3.23×10⁵~1.28×10⁶ units/mg.
Purity	> 90% by SDS-PAGE.
Endotoxin	< 0.1 EU/μg of the protein by LAL method.
Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Species	Human
Calculated MW	18.23 kDa

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.