Overview
Product name | Recombinant Human ISG15 Protein |
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Catalog No. | RP00061 |
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Description | Recombinant Human ISG15 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Gly2-Gly157) of human ISG15 (Accession #NP_005092.1). |
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Purity | > 97% by SDS-PAGE. |
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Endotoxin | < 1.0 EU/μg of the protein by LAL method. |
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Formulation | Lyophilized from a 0.22 μm filtered solution of 50mM HEPES, 100mM NaCl, pH 7.5.Contact us for customized product form or formulation. |
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Species | Human |
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Calculated MW | 17.01 kDa |
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Background
The protein is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted.
Product information
Synonym | G1P2; IFI15; IMD38; IP17; UCRP; hUCRP; ISG15; IFI15; IMD38; IP17; UCRP; hUCRP |
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Expression Host | E. coli |
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Tag | No tag |
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Swiss-Prot | |
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Gene ID | |
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Usage guide
Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.