Overview
| Product name | Recombinant Human IgG2A Protein |
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| Catalog No. | RPT0005 |
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| Description | Recombinant Human IgG2A Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Glu99-Lys326) of human IgG2A (Accession #) fused with and NO-tag at the C-terminus. |
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| Bio-Activity | Measured by its binding ability in a functional ELISA.Immobilized Human CD32a (Catalog: RP00081) at 2 μg/mL (100 μL/well) can bind Human IgG2 (Catalog: RPT0005) with a linear range of 1.2-338 ng/mL.
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| Purity | > 97% by SDS-PAGE. |
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| Endotoxin | <0.1EU/μg |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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| Species | Human |
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| Calculated MW | 25.72 kDa |
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Background
Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens
Product information
| Synonym | IGHG2; Immunoglobulin heavy constant gamma 2; IgG2 |
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| Expression Host | HEK293 cells |
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| Tag | NO-tag |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
