Overview
| Product name | Recombinant Human Insulin Receptor/INSR/CD220 Protein |
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| Catalog No. | RP01146 |
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| Description | Recombinant Human Insulin Receptor/INSR/CD220 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Lys956) of human Insulin Receptor (Accession #NP_000199.2) fused with a 6×His tag at the C-terminus. |
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| Purity | > 95 % by SDS-PAGE. |
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| Endotoxin | < 1.0 EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution PBS, pH 7.4.Contact us for customized product form or formulation. |
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| Species | Human |
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| Calculated MW | 83 kDa(α chain), 24 kDa(ECD of β subunit) |
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Background
This protein is a member of the receptor tyrosine kinase family of proteins. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form a heterotetrameric receptor. Binding of insulin or other ligands to this receptor activates the insulin signaling pathway, which regulates glucose uptake and release, as well as the synthesis and storage of carbohydrates, lipids and protein. Mutations in this gene underlie the inherited severe insulin resistance syndromes including type A insulin resistance syndrome, Donohue syndrome and Rabson-Mendenhall syndrome. Alternative splicing results in multiple transcript variants.
Product information
| Synonym | CD220; HHF5; INSR; CD220; HHF5; insulin receptor |
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| Expression Host | HEK293 cells |
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| Tag | C-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
