Overview
| Product name | Recombinant Human Kallikrein-11/KLK11 Protein |
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| Catalog No. | RP00149 |
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| Description | Recombinant Human Kallikrein-11/KLK11 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Asn250) of human KLK11/Kallikrein-11 (Accession #NP_006844.1) fused with a 6×His tag at the C-terminus. |
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| Bio-Activity | Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5, 5’Dithio-bis (2-nitrobenzoic acid) (DTNB). The specific activity is >141.40 pmol/min/μg.
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| Purity | > 92% by SDS-PAGE. |
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| Endotoxin | < 0.1 EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of 20mM Tris, 150mM NaCl, pH 8.0.Contact us for customized product form or formulation. |
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| Species | Human |
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| Calculated MW | 28.14 kDa |
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Background
kallikrein-related peptidase 11 (KLK11), also known as hippostasin, trypsin-like serine protease and PRSS20, is a member of human tissue kallikrein family. It is a subgroup of serine proteases with diverse physiological functions, which is implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers.The KLK11 gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19.Two alternatively spliced forms exist, resulting in 250 (isoform 1) and 282 (isoform 2) amino acid sequences, respectively. Isoform 1 is predominantly expressed in brain whereas isoform 2 is preferentially expressed in prostate. KLK11 is a novel marker for ovarian and prostate cancer carcinomas. Recombinant human KLK11, after being activated by thermolysin, is active against a thioester substrate. This activity can be inhibited by AEBSF dichloroisocoumarin, and aprotinin.
Product information
| Synonym | KLK11; PRSS20; TLSP |
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| Expression Host | HEK293 cells |
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| Tag | C-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
