Overview
| Product name | Recombinant Human Kininogen-1/KNG1 Protein |
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| Catalog No. | RP01026 |
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| Description | Recombinant Human Kininogen-1/KNG1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln 19 - Ser 427 ) of human Kininogen-1 (Accession #NP_000884) fused with a 6×His tag at the C-terminus. |
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| Bio-Activity | Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC. The IC50 value approximately is 1 nM.
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| Purity | > 97% by SDS-PAGE. |
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| Endotoxin | < 0.1 EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
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| Species | Human |
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| Calculated MW | 46.66 kDa |
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Background
Kininogen-1 (KNG1) is also known as high molecular weight kininogen, Alpha-2-thiol proteinase inhibitor, Fitzgerald factor, Williams-Fitzgerald-Flaujeac factor, which can be cleaved into the following 6 chains:Kininogen-1 heavy chain, T-kinin, Bradykinin, Lysyl-bradykinin, Kininogen-1 light chain, Low molecular weight growth-promoting factor. Kininogen-1 is a secreted protein which contains three cystatin domains. HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII. As with many other coagulation proteins, the protein was initially named after the patients in whom deficiency was first observed.Patients with HWMK deficiency do not have a hemorrhagic tendency, but they exhibit abnormal surface-mediated activation of fibrinolysis.
Product information
| Synonym | BDK; BK; KNG; KNG1; BK; KNG |
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| Expression Host | HEK293 cells |
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| Tag | C-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
