| Product name | Recombinant Human Klotho beta/KLB Protein |
|---|---|
| Catalog No. | RP02210 |
| Description | Recombinant Human Klotho beta/KLB Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met30-Thr983) of Human Klotho beta/KLB (Accession #NP_783864.1) fused with a his tag at the C-terminus. |
| Bio-Activity | Immobilized Human Klotho at 1 μg/mL(100 μL/Well).Dose response curve for Anti-Klotho Ab.1 with the EC50 of 0.4 μg/ml determined by ELISA. Immobilized Human Klotho at 2μg/ml(100 μL/Well).Dose response curve for Anti-Klotho Ab. 2 with the EC50 of 20.5 ng/mL determined by ELISA. |
| Purity | > 95% by SDS-PAGE. |
| Endotoxin | < 1 EU/μg of the protein by LAL method. |
| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| Species | human |
| Calculated MW | 110.25 kDa |
| Synonym | KLB; Beta-klotho; BKL; BetaKlotho; Klotho beta-like protein |
|---|---|
| Expression Host | HEK293 cells |
| Tag | C-His |
| Swiss-Prot | |
| Gene ID |
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
|---|---|
| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
* For research use only. Not for therapeutic or diagnostic purposes.
