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Recombinant Human LAG-3/CD223 Protein (RP00193)

Bioactivityvalidated

Datasheet

Overview

Product nameRecombinant Human LAG-3/CD223 Protein
Catalog No.RP00193
DescriptionRecombinant Human LAG-3/CD223 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Leu23-Leu450) of human LAG-3/CD223 (Accession #NP_002277.4) fused with a 6×His tag at the C-terminus.
Bio-Activity1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human LAG3 at 2 μg/mL (100 μL/well) can bind Anti-LAG3 antibody with a linear range of 13-40 ng/mL.
2.Measured by its binding ability in a functional ELISA. Immobilized Human FGL1 at 5 μg/mL (100 μL/well) can bind recombinant Human LAG3, the EC50 of Human LAG3 is 0.32 μg/mL.
Purity> 95% by SDS-PAGE.
Endotoxin< 0.1 EU/μg of the protein by LAL method.
FormulationLyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
SpeciesHuman
Calculated MW47.02 kDa
SynonymLAG3; CD223
Expression HostHEK293 cells
TagC-His
Swiss-Prot
Gene ID
StorageStore at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
ReconstitutionCentrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.

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