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Recombinant Human LILRB4/ILT-3/CD85k Protein (RP01185)

Bioactivityvalidated

Datasheet

Overview

Product nameRecombinant Human LILRB4/ILT-3/CD85k Protein
Catalog No.RP01185
DescriptionRecombinant Human LILRB4/ILT-3/CD85k Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln22-Glu259) of human LILRB4/CD85k/ILT3 (Accession #NP_001265356.2) fused with a 6×His tag at the C-terminus.
Bio-Activity1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human LILRB4 at 5 μg/mL (100 μL/well) can bind Recombinant Human ANGPTL7 with a linear range of 0.12-0.5 μg/mL.
2.Measured by its binding ability in a functional ELISA. Immobilized Human LILRB4 Protein at 1μg/mL (100 μL/well) can bind LILRB4 Rabbit pAb with a linear range of 0.03-1.4 ng/mL.
Purity> 92% by SDS-PAGE.
Endotoxin< 0.1 EU/μg of the protein by LAL method.
FormulationLyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
SpeciesHuman
Calculated MW27.10 kDa
SynonymLILRB4; CD85K; ILT-3; ILT3; LIR-5; LIR5
Expression HostHEK293 cells
TagC-His
Swiss-Prot
Gene ID
StorageStore at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
ReconstitutionCentrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.

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