Overview
| Product name | Recombinant Human LMP2(HLA-A*11:01) Tetramer Protein |
|---|
| Catalog No. | RP02696 |
|---|
| Description | Recombinant Human LMP2(HLA-A*11:01) Tetramer Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal, tetramer is assembled by biotinylated monomer and streptavidin.；It contains Gly25-Thr305(HLA-A*11:01), Ile21-Met119(B2M) and SSCSSCPLTK peptide. |
|---|
| Purity | > 95% as determined by Tris-Bis PAGE；> 95% as determined by HPLC |
|---|
| Endotoxin | Less than 1EU per μg by the LAL method. |
|---|
| Species | Human |
|---|
| Calculated MW | 258 kDa |
|---|
Background
The immunoproteasome, having been linked to neurodegenerative diseases and hematological cancers, has been shown to play an important role in MHC class I antigen presentation. The development of molecular probes that selectively inhibit the major catalytic subunit, LMP2, of the immunoproteasome, LMP2-rich cancer cells compared to LMP2-deficient cancer cells are more sensitive to growth inhibition by the LMP2-specific inhibitor, implicating an important role of LMP2 in regulating cell growth of malignant tumors that highly express LMP2.
Product information
| Synonym | MHC; RMF; LMP2; LMP-2; Macropain chain 7; Proteasome chain 7; PSMB9; RING12 |
|---|
| Expression Host | HEK293 cells |
|---|
| Tag | C-His&Avi |
|---|
| Swiss-Prot | |
|---|
Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
|---|
| Reconstitution | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
|---|
* For research use only. Not for therapeutic or diagnostic purposes.
