Recombinant Human Latent TGF-beta 1 (C33S) Protein (RP00161)

Product name	Recombinant Human Latent TGF-beta 1 (C33S) Protein
Catalog No.	RP00161
Description	Recombinant Human Latent TGF-beta 1 (C33S) Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Leu30-Ser390(C33S)) of human TGF-beta 1 (Accession #NP_000651.3) fused with an 8×His tag at the N-terminus.
Bio-Activity	1.Measured by its ability to inhibit cell proliferation of TF-1 human erythroleukemic cells. The ED₅₀ for this effect is typically 0.5-2 ng/mL, corresponding to a specific activity of 5.0×10⁵~2.0×10⁶ units/mg. 2.Measured by its binding ability in a functional ELISA. Immobilized recombinant human TGFB1 at 2 μg/mL (100 μL/well) can bind recombinant human TGFBR2 with a linear range of 1-5 ng/mL. 3.Measured by its ability to inhibit the IL-4(Catalog: RP01161)-dependent proliferation of HT-2 mouse T cells. The ED₅₀ for this effect is 9.75-39 ng/mL, corresponding to a specific activity of 2.6×10⁴~1.0×10⁵ units/mg.
Purity	> 95% by SDS-PAGE.
Endotoxin	< 0.1 EU/μg of the protein by LAL method.
Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Species	Human
Calculated MW	42.09 kDa

Synonym	TGFB1; CED; DPD1; LAP; TGFB; TGFbeta; transforming growth factor beta-1; TGF-beta 1; CED; DPD1; LAP; TGFB; TGFbeta; TGF-β
Expression Host	HEK293 cells
Tag	N-His
Swiss-Prot
Gene ID

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile 4 mM HCl. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.