Overview
| Product name | Recombinant Human MADCAM1 Protein |
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| Catalog No. | RP02808 |
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| Description | Recombinant Human MADCAM1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln19-Gln317) of human MADCAM1 (Accession #NP_570116.2) fused with a 6×His tag at the C-terminus. |
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| Bio-Activity | Immobilized Human MADCAM1, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-MADCAM1 Antibody, hFc Tag with the EC50 of 7.7ng/ml determined by ELISA.
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| Purity | > 95% as determined by HPLC |
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| Endotoxin | <1EU/μg |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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| Species | Human |
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| Calculated MW | 32.5 kDa |
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Background
Mucosal addressin cell adhesion molecule-1 (MAdCAM-1) contributes to the recruitment of donor T cells into the mucosal tissues of the recipient after allogeneic hematopoietic stem cell transplantation (aHSCT). The aim of our study was to determine whether selected single nucleotide polymorphisms (SNPs) of the MADCAM1 gene are associated with development of serious complications after aHSCT.
Product information
| Synonym | MAdCAM-1; MADCAM1; MACAM1 |
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| Expression Host | HEK293 cells |
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| Tag | C-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
