Overview
| Product name | Recombinant Human MANF Protein |
|---|
| Catalog No. | RP00005 |
|---|
| Description | Recombinant Human MANF Protein is produced by E. coli expression system. The target protein is expressed with sequence (Leu25-Leu182) of human MANF (Accession #NP_006001.4). |
|---|
| Purity | > 95% by SDS-PAGE. |
|---|
| Endotoxin | < 0.1 EU/μg of the protein by LAL method. |
|---|
| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
|---|
| Species | Human |
|---|
| Calculated MW | 18.16 kDa |
|---|
Background
The protein is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of this protein increases susceptibility to ER stress-induced death and results in cell proliferation. Activity of this protein is important in promoting the survival of dopaminergic neurons. The presence of polymorphisms in the N-terminal arginine-rich region, including a specific mutation that changes an ATG start codon to AGG, have been reported in a variety of solid tumors; however, these polymorphisms were later shown to exist in Normal tissues and are thus no longer thought to be tumor-related.
Product information
| Synonym | MANF; ARMET; ARP |
|---|
| Expression Host | E. coli |
|---|
| Tag | No tag |
|---|
| Swiss-Prot | |
|---|
| Gene ID | |
|---|
Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
|---|
| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
|---|
* For research use only. Not for therapeutic or diagnostic purposes.
