Overview
| Product name | Recombinant Human MHC class I polypeptide-related sequence A/MIC-A Protein |
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| Catalog No. | RP00172 |
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| Description | Recombinant Human MHC class I polypeptide-related sequence A/MIC-A Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Gln308) of human MICA (Accession #NP_000238.1) fused with a 6×His tag at the C-terminus. |
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| Bio-Activity | Measured by its binding ability in a functional ELISA. Immobilized Human MICA Protein at 2 μg/mL (100 μL/well) can bind NKG2D with a linear range of 0.61-14.3 ng/mL.
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| Purity | > 95% by SDS-PAGE. |
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| Endotoxin | < 0.1 EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
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| Species | Human |
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| Calculated MW | 35.88 kDa |
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Background
MHC class I chain-related molecules A (MICA) is one of the genes in the HLA class I region, which belongs to MHC class I family. It is the member of the non-classical class I family that displays the greatest degree of polymorphism. The MICA protein product is expressed on the cell surface, although unlike canonical class I molecules it does not seem to associate with beta-2-microglobulin. It is a ligand for the NKG2-D type II integral membrane protein receptor. The protein functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells. Variations in this gene have been associated with susceptibility to psoriasis 1 and psoriatic arthritis, and the shedding of MICA-related antibodies and ligands is involved in the progression from monoclonal gammopathy of undetermined significance to multiple myeloma.
Product information
| Synonym | MICA; MIC-A; PERB11.1; MHC class I polypeptide-related sequence A; MIC-A; PERB11.1 |
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| Expression Host | HEK293 cells |
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| Tag | C-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
