Overview
Product name | Recombinant Human MME/CD10 Protein |
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Catalog No. | RP02826 |
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Description | Recombinant Human MME/CD10 Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Tyr52-Trp750) of human MME/CD10 (Accession #NP_000893.2) fused with a 6×His tag at the C-terminus. |
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Bio-Activity | Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK (Dnp)-OH, (R&D Systems, Catalog # ES005). The specific activity is >1, 500 pmoles/min/μg.
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Purity | > 95% by SDS-PAGE. |
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Endotoxin | <1EU/μg |
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Formulation | Lyophilized from a 0.22 μm filtered solution of 20mM MES, 100mM NaCl, 1mM ZnCl2, 10%glycerol, pH 6.5. |
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Species | Human |
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Calculated MW | 82.2 kDa |
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Background
The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alters the behavior of the cell. Some CD proteins do not take part in the cell signal process but have other functions such as cell adhesion. The cluster of differentiation 10 (CD10), also known as Neprilysin and neutral endopeptidase, is a member of the CD system. CD10 is a zinc-dependent metalloprotease enzyme that had the function to degrade some small secreted peptides such as the amyloid beta-peptide. It exists as a membrane-bound protein and has a high concentration in kidney and lung tissues. Mutations in the CD10 gene can induce the familial forms of Alzheimer's disease, providing strong evidence for the protein's association with the Alzheimer's disease process. CD10 is also associated with other biochemical processes.
Product information
Synonym | MME; EPN |
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Expression Host | HEK293 Cells |
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Tag | C-His |
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Swiss-Prot | |
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Gene ID | |
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Usage guide
Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.