Overview
| Product name | Recombinant Human Mature GDNF Protein |
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| Catalog No. | RP00835 |
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| Description | Recombinant Human GDNF Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ser78-Ile211) of human GDNF (Accession #P39905). |
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| Bio-Activity | Measured in a cell proliferation assay using SH-SY5Y cells. The ED50 for this effect is 1.82-7.26 ng/mL in the presence of Recombinant Human GFR alpha ‑1 (Catalog # RP01970), corresponding to a specific activity of 1.38×105 ~5.49×105 units/mg.
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| Purity | ≥ 95 % as determined by SDS-PAGE |
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| Endotoxin | < 0.01 EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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| Species | Human |
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| Calculated MW | 15.07 kDa |
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Background
Glial cell line-derived neurotrophic factor(GDNF) is an important member of the GDNF family of ligands(GFL). The GDNF family of ligands is comprised by four neurotrophic factors: glial cell line-derived neurotrophic factor (GDNF), neurturin (NRTN), artemin (ARTN), and persephin (PSPN). It has been found that GFLs play a role in a number of biological processes including cell survival, neurite outgrowth, cell differentiation and cell migration. As the founding member, GDNF plays a key role in the promotion of the survival of dopaminergic neurons. GDNF is a highly conserved neurotrophic factor. The recombinant form of this protein also promotes the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. GDNF also regulates kidney development and spermatogenesis, and it affects alcohol consumption. It has been shown that GDNF results in two Parkinson's disease clinical trial and in a number of animal trials. It has been taken as a potent survival factor for central motoneurons.
Product information
| Synonym | GDNF; Glial cell line-derived neurotrophic factor; hGDNF; Astrocyte-derived trophic factor; ATF |
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| Expression Host | E. coli |
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| Tag | NO-Tag |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
