Overview
| Product name | Recombinant Human/Mouse/Rat mature Activin A/INHBA Protein |
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| Catalog No. | RP01782S |
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| Description | Recombinant Human/Mouse/Rat mature Activin A/INHBA Protein is produced by CHO cells expression system. The target protein is expressed with sequence (Gly311-Ser426) of Human/Mouse/Rat mature Activin A/INHBA (Accession #NP_002183.1) fused with a His tag at the N-terminus. |
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| Bio-Activity | Measured by its ability to inhibit proliferation of MPC-11 cells. The ED50 for this effect is 3.215-12.86 ng/mL, corresponding to a specific activity of 7.78×104~3.11×105 units/mg.
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| Endotoxin | < 0.01 EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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| Species | Human |
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| Calculated MW | 12.98 kDa |
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Background
The inhibin beta A subunit joins the alpha subunit to form a pituitary FSH secretion inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumor-suppressor activity. In addition, serum levels of inhibin have been shown to reflect the size of granulosa-cell tumors and can therefore be used as a marker for primary as well as recurrent disease. Because expression in gonadal and various extragonadal tissues may vary severalfold in a tissue-specific fashion, it is proposed that inhibin may be both a growth/differentiation factor and a hormone. Furthermore, the beta A subunit forms a homodimer, activin A, and also joins with a beta B subunit to form a heterodimer, activin AB, both of which stimulate FSH secretion. Finally, it has been shown that the beta A subunit mRNA is identical to the erythroid differentiation factor subunit mRNA and that only one gene for this mRNA exists in the human genome.
Product information
| Synonym | EDF; FRP; INHBA; Activin A |
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| Expression Host | CHO Cells |
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| Tag | No-Tag |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifμge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
