Recombinant Human NKAT-1/KIR2DL1/CD158a Protein (RP01431)

Overview

Product name	Recombinant Human NKAT-1/KIR2DL1/CD158a Protein
Catalog No.	RP01431
Description	Recombinant Human NKAT-1/KIR2DL1/CD158a Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (His22-Arg242) of human KIR2DL1 (Accession #NP_055033.2) fused with a 6×His，Avi tag at the C-terminus.
Bio-Activity	Measured by its binding ability in a functional ELISA.Immobilized Human KIR2DL1 at 1μg/mL (100 μL/well) can bind KIR2DL1 Rabbit pAb with a linear range of 1-99 ng/mL.
Purity	> 95% by SDS-PAGE.
Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Species	Human
Calculated MW	26.87 kDa

Killer cell immunoglobulin-like receptor 2DL1 or KIR2DL1 is an inhibitory Natural Killer cell immunoglobulin-like receptor with two extracellular immunoglobulin domains. KIR2DL1 is a member of the Killer cell immunoglobulin-like receptor family whose members are classified by the number of the extracellular immunoglobulin domains and the length of the cytoplasm domain. KIR2DL1 is a transmembrane glycoprotein expressed by natural killer cells and subsets of T cells. KIR2DL1 down-regulates the cytotoxicity of NK cells upon recognition of specific class I major histocompatibility complex (MHC) molecules on target cells. It has been reported that the KIR2DL1 is bound to its class I MHC ligand, HLA-Cw4. The KIR2DL1-HLA-Cw4 interface exhibits charge and shape complementarity. Specificity is mediated by a pocket in KIR2DL1 that hosts the Lys80 residue of HLA-Cw4. Many residues conserved in HLA-C and KIR2DL receptors make different interactions in KIR2DL1-HLA-Cw4 and a previously reported KIR2DL2-HLA-Cw3 complex. A dimeric aggregate of KIR-HLA-C complexes was observed in one KIR2DL1-HLA-Cw4 crystal.

Product information

Synonym	KIR2DL1; CD158A; KIR-K64; KIR221; NKAT; NKAT-1; NKAT1; p58.1
Expression Host	HEK293 cells
Tag	C-His&Avi
Swiss-Prot
Gene ID

Usage guide

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.